ナノ粒子を用いた分化誘導療法による新たな神経芽腫治療法の開発

Outline of Final Research Achievements

For high-risk neuroblastoma, we planned to develop a treatment method using nanoparticles containing isotretinoic acid and bound with MIBG. In the first year of the study, we were able to create nanoparticles encapsulating retinoic acid. Next, an experiment was performed using a mouse neuroblastoma cell line. By co-culturing with nanoparticles encapsulating retinoic acid, NB2a differentiation was promoted and morphological changes were confirmed. Since it is difficult to bind MIBG to nanoparticles, we investigated the role of retinoic acid-embedded nanoparticles in vivo. We examined the growth and histopathological differentiation of NB2a subcutaneous tumors inoculated into mice, but could not establish an experimental system. Based on this study, we plan to carry out the study again with other neuroblastoma cell lines.