Effect of harringtonine on apoptotic cell death and cell cycle progression in human leukemia HL60 cells

Y. Liu -p.; T. Ueda; A. Yoshida; H. Iwasaki; T. Nakamura

Effect of harringtonine on apoptotic cell death and cell cycle progression in human leukemia HL60 cells

Y. Liu -p.
, T. Ueda
, A. Yoshida
, H. Iwasaki
, T. Nakamura

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The mechanism of cell killing induced by haningtonine (HT) was investigated in HL60 cells by metabolic labeling studies, light and transmission electron microscopy, agarose gel electrophoresis of DNA and flow cytometry. At concentrations higher than 0.02 μM HT showed significant inhibition on cell growth and protein and DNA synthesis. Following exposure to HT, typical apoptotic cell death was observed from 1.5 to 4 h and it increased in both dose- and time-dependent fashion. This process was prevented by an intracellular calcium ion chelator. In addition, at 0.04 μM of HT the nonapoptotic cells delayed the progression of cell cycle through S and G2 phase and finally arrested in G₁ phase. These results are important to understand the mechanism of action of HT and to improve the effect of HT in cancer chemotherapy.

Original language	English
Pages (from-to)	1509-1515
Number of pages	7
Journal	ANTICANCER RESEARCH
Volume	14
Issue number	4 A
State	Published - 1994

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptosis
Calcium ion
Cell cycle
Flow cytometry
Harringtonine
Morphology

Cite this

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title = "Effect of harringtonine on apoptotic cell death and cell cycle progression in human leukemia HL60 cells",

abstract = "The mechanism of cell killing induced by haningtonine (HT) was investigated in HL60 cells by metabolic labeling studies, light and transmission electron microscopy, agarose gel electrophoresis of DNA and flow cytometry. At concentrations higher than 0.02 μM HT showed significant inhibition on cell growth and protein and DNA synthesis. Following exposure to HT, typical apoptotic cell death was observed from 1.5 to 4 h and it increased in both dose- and time-dependent fashion. This process was prevented by an intracellular calcium ion chelator. In addition, at 0.04 μM of HT the nonapoptotic cells delayed the progression of cell cycle through S and G2 phase and finally arrested in G1 phase. These results are important to understand the mechanism of action of HT and to improve the effect of HT in cancer chemotherapy.",

keywords = "Apoptosis, Calcium ion, Cell cycle, Flow cytometry, Harringtonine, Morphology",

author = "\{Liu -p.\}, Y. and T. Ueda and A. Yoshida and H. Iwasaki and T. Nakamura",

year = "1994",

language = "英語",

volume = "14",

pages = "1509--1515",

number = "4 A",

}

TY - JOUR

T1 - Effect of harringtonine on apoptotic cell death and cell cycle progression in human leukemia HL60 cells

AU - Liu -p., Y.

AU - Ueda, T.

AU - Yoshida, A.

AU - Iwasaki, H.

AU - Nakamura, T.

PY - 1994

Y1 - 1994

N2 - The mechanism of cell killing induced by haningtonine (HT) was investigated in HL60 cells by metabolic labeling studies, light and transmission electron microscopy, agarose gel electrophoresis of DNA and flow cytometry. At concentrations higher than 0.02 μM HT showed significant inhibition on cell growth and protein and DNA synthesis. Following exposure to HT, typical apoptotic cell death was observed from 1.5 to 4 h and it increased in both dose- and time-dependent fashion. This process was prevented by an intracellular calcium ion chelator. In addition, at 0.04 μM of HT the nonapoptotic cells delayed the progression of cell cycle through S and G2 phase and finally arrested in G1 phase. These results are important to understand the mechanism of action of HT and to improve the effect of HT in cancer chemotherapy.

AB - The mechanism of cell killing induced by haningtonine (HT) was investigated in HL60 cells by metabolic labeling studies, light and transmission electron microscopy, agarose gel electrophoresis of DNA and flow cytometry. At concentrations higher than 0.02 μM HT showed significant inhibition on cell growth and protein and DNA synthesis. Following exposure to HT, typical apoptotic cell death was observed from 1.5 to 4 h and it increased in both dose- and time-dependent fashion. This process was prevented by an intracellular calcium ion chelator. In addition, at 0.04 μM of HT the nonapoptotic cells delayed the progression of cell cycle through S and G2 phase and finally arrested in G1 phase. These results are important to understand the mechanism of action of HT and to improve the effect of HT in cancer chemotherapy.

KW - Apoptosis

KW - Calcium ion

KW - Cell cycle

KW - Flow cytometry

KW - Harringtonine

KW - Morphology

UR - https://www.scopus.com/pages/publications/0028029998

M3 - 学術原著論文

C2 - 7979177

AN - SCOPUS:0028029998

SN - 0250-7005

VL - 14

SP - 1509

EP - 1515

JO - ANTICANCER RESEARCH

JF - ANTICANCER RESEARCH

IS - 4 A

ER -

Effect of harringtonine on apoptotic cell death and cell cycle progression in human leukemia HL60 cells

Abstract

UN SDGs

Keywords

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